OFFICE OF NAVAL RESEARCH CONTRACT N00014-88-C-0118 TECHNICAL REPORT 94-03 IMMUNE RESPONSES TO MHC HOMOZYGOUS LYMPHOID CELLS IN MURINE Fl HYBRID RECIPIENTS: IMPLICATIONS FOR TRANSFUSION ASSOCIATED GRAFT-VERSUS HOST DISEASE
نویسندگان
چکیده
Graft-versus-host disease(GVHD) is currently encountered following bone marrow transplantation and transfusion. GVHD associated with transfusion(TA-GVHD) in apparently immunocompetent recipients has been recently reported with increasing frequency. A consistent finding in many of these cases is that the recipient received blood from a donor homozygous for one of the the recipient's HLA haplotypes. However the observed frequency of TA-GVHD is much lower than the estimated probability of this donor/recipient combination. The potential role of recipient immune responses in controlling TA-GVHD was investigated using an analogous murine model where GVHD is induced by the injection of parental lymphoid cells into unirradiated Fi hybrid recipients. The effect of various immune manipulations of the recipient on GVHD induction was assessed by determining the number of donor lymphoid cells required to induce GVHD responses. While depletion of recipient CD4+ cells increased the number of donor cells needed to induce GVHD, depletion of recipient CD8+ and natural killer cells resulted in fewer donor cells being needed to induce a GVHD response. These studies suggest a central role for functioning recipient CD8 and natural killer(NK) cells in the downregulation of TA-GVHD development in recipients who receive blood from a haploidentical donor.
منابع مشابه
Immune responses to major histocompatibility complex homozygous lymphoid cells in murine F1 hybrid recipients: implications for transfusion-associated graft-versus-host disease.
Graft-versus-host disease (GVHD) is currently encountered after bone marrow transplantation and transfusion. GVHD associated with transfusion (TA-GVHD) in apparently immunocompetent recipients has been recently reported with increasing frequency. A consistent finding in many of these cases is that the recipient received blood from a donor homozygous for one of the recipient's HLA haplotypes. Ho...
متن کاملOffice of Naval Research Contract N00014-88-c-0118 Technical Report 94-01 Effects of Stroma Free Hemoglobin on Blood Pressure and Renal Function in the Hypotensive Rat: Potential Role of Nitric Oxide Inactivation by Hemoglobin
متن کامل
Office of Naval Research Contract N00014-94-c-0149 Technical Report 97-03 Effects of Cardiopulmonary Bypass on Hemostasis
متن کامل
Comparison of Functional Competence of Umbilical Cord and Adult Peripheral Blood Dendritic Cells in Allogenic Mixed Leukocyte Reaction
Background: Dendritic cells (DCs) are the most potent stimulators of primary T cell responses and play a key role in immune reactions after stem cell transplantation. Very little is known about the cord blood (CB) dendritic cells and their potential involvement in the low incidence and lower severity of acute graft-versus-host disease after CB transplantation. Objectives: The aim of this stud...
متن کاملAllogeneic marrow stromal cells are immune rejected by MHC class I- and class II-mismatched recipient mice.
It has been suggested that marrow stromal cells (MSCs) may be immunoprivileged and can engraft in allogeneic recipients with intact immune systems. We determined if the implantation of murine MSCs engineered to release erythropoietin (Epo) would be feasible in major histocompatibility complex (MHC)-mismatched allogeneic mice without immunosuppression, and we monitored hematocrit (Hct) as a repo...
متن کامل